Ontogenetic shift in feeding habits and trophic levels of bluefin tuna (Thunnus thynnus) in Mediterranean

Abstract

Possible changes in diet and trophic levels in relation to size of Mediterranean bluefin tuna, Thunnus thynnus, were investigated using labelled carbon (d13C) and nitrogen (d15N) stable isotopes. Samples were obtained from two locations in the southern Tyrrhenian Sea (Western Mediterranean Sea) in May and October 2004. The d13C and d15N analyses revealed at least three significant isotopic groups [small juveniles (0.7– 2.2 kg), sub-adults (15–50 kg) and adults (70 to 225 kg)]. d13C was negatively dependent on weight, while d15N was positively dependent on weight [TW ¼ 8.2 (±0.16) + 0.03 (± 0.0) *d15N (n ¼ 49; r ¼ 0.91; P < 0.001)]. Different prey contribution to the diet was highlighted for each class. The diet of juveniles comprised zooplankton, small pelagic fish and some coastal fish; sub-adults relied on medium pelagic fish, shrimps and cephalopods, and adults relied mainly on cephalopods and larger fish. The trophic level (TL) of tunas belonging to each size class was closely correlated to weight, starting from ca 3.0 TL for Group I and reaching 4.4–4.8 TL for the giants. Bluefin tuna, from small juveniles to giants, showed a shift in feeding preferences due to different use of habitats and food items as a function of the life stage.