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GIANCARLO MOSCHETTI

Introduction of a novel pathway for IAA biosynthesis to rhizobia alters vech root nodule developmt

  • Authors: Camerini, S; Senatore, B; Lonardo, E; Imperlini, E; Bianco, C; Moschetti, G; Rotino, GL; Campion, B; Defez, R
  • Publication year: 2008
  • Type: Articolo in rivista (Articolo in rivista)
  • OA Link: http://hdl.handle.net/10447/36860

Abstract

We introduced into Rhizobium leguminosarum bv. viciae LPR1105 a new pathway for the biosynthesis of the auxin, indole-3-acetic acid (IAA), under the control of a stationary phase-activated promoter active both in free-liv- ing bacteria and bacteroids. The newly introduced genes are the iaaM gene from Pseudomonas savastanoi and the tms2 gene from Agrobacterium tumefaciens. Free-living bacteria harbouring the promoter-iaaMtms2 construct release into the growth medium 14-fold more IAA than the wild-type parental strain. This IAA overproducing R. l. viciae, the RD20 strain, elicits the development of vetch root nodules containing up to 60-fold more IAA than nodules infected by the wild-type strain LPR1105. Vetch root nodules derived from RD20 are fewer in number per plant, heavier in terms of dry weight and show an enlarged and more active meri- stem. A signiWcant increase in acetylene reduction activity was measured in nodules elicited in vetch by RD20.