NiII, and ZnII Schiff Base Complexes: Telomeric G-quadruplex Stabilizers
- Authors: Marrone, A; Bonsignore, R; Terenzi, A; Spinello, A; Gentile, C; Martorana, A; Almerico, AM; Lauria, A; Barone, G
- Publication year: 2014
- Type: Proceedings
- OA Link: http://hdl.handle.net/10447/98673
Abstract
Recently, NiII and ZnII metal complexes of the ligand Salpyrim have been synthesized and characterized. Their affinity for wild-type h-Telo G-quadruplex DNA and for calf thymus DNA was investigated by UV absorption spectroscopy, circular dichroism and viscometry. The data collectively suggest that both complexes bind effectively to G-quadruplexes by direct end-stacking, stabilizing the oligonucleotide secondary structure. The two complexes are also typical B-DNA intercalators. Remarkably, their binding constants, Kb, with the G4s structures are about 10 fold higher than those with B-DNA, highlighting the selectivity. Experiments to evaluate the biological activity of the two complexes against MCF7 and HeLa cancer cell lines are currently ongoing.