Melt Processed PCL/PEG Scaffold with Discrete Pore Size Gradient for Selective Cellular Infiltration
- Autori: Scaffaro, R.; Lopresti, F.; Botta, L.; Rigogliuso, S.; Ghersi, G.
- Anno di pubblicazione: 2016
- Tipologia: Articolo in rivista (Articolo in rivista)
- Parole Chiave: functionally graded scaffold; interface tissue engineering; melt mixing; particulate leaching; PCL multilayer scaffold; pore size gradient; Organic Chemistry; Materials Chemistry2506 Metals and Alloys; Polymers and Plastics; Chemical Engineering (all)
- OA Link: http://hdl.handle.net/10447/181377
Abstract
In order to develop scaffold able to mimic the natural gradient properties of tissues, biphasic and triphasic approaches were adopted. In this work, polycaprolactone/polyethylene glycol (PCL/PEG) scaffolds were prepared by using a combination of melt mixing and selective leaching without harmful solvents. The method permitted to develop three-layer scaffolds with high control of porosity and pore size. The mechanical properties were evaluated under physiological condition in order to simulate the real conditions of work. Co-culture of osteoblastic and fibroblastic mice cells were carried out in order to study the differential cellular permeation through the different pore size layers.