Effects of Plant Sterols or β-Cryptoxanthin at Physiological Serum Concentrations on Suicidal Erythrocyte Death
- Authors: Alvarez-Sala, Andrea; López-GarcÃa, Gabriel; Attanzio, Alessandro*; Tesoriere, Luisa; Cilla, Antonio; Barberá, Reyes; AlegrÃa, Amparo
- Publication year: 2018
- Type: Articolo in rivista (Articolo in rivista)
- Key words: eryptosis; hemolysis; oxidative stress; phytosterol; β-cryptoxanthin; Beta-Cryptoxanthin; Cells, Cultured; Cholesterol; Eryptosis; Erythrocytes; Glutathione; Hemolysis; Humans; Oxidative Stress; Phytosterols; Sitosterols; Stigmasterol; tert-Butylhydroperoxide; Chemistry (all); Agricultural and Biological Sciences (all)
- OA Link: http://hdl.handle.net/10447/289236
Abstract
The eryptotic and hemolytic effects of a phytosterol (PS) mixture (β-sitosterol, campesterol, stigmasterol) or β-cryptoxanthin (β-Cx) at physiological serum concentration and their effect against oxidative stress induced by tert-butylhydroperoxide (tBOOH) (75 and 300 μM) were evaluated. β-Cryptoxanthin produced an increase in eryptotic cells, cell volume, hemolysis, and glutathione depletion (GSH) without ROS overproduction and intracellular Ca2+influx. Co-incubation of both bioactive compounds protected against β-Cx-induced eryptosis. Under tBOOH stress, PS prevented eryptosis, reducing Ca2+influx, ROS overproduction and GSH depletion at 75 μM, and hemolysis at both tBOOH concentrations. β-Cryptoxanthin showed no cytoprotective effect. Co-incubation with both bioactive compounds completely prevented hemolysis and partially prevented eryptosis as well as GSH depletion induced by β-Cx plus tBOOH. Phytosterols at physiological serum concentrations help to prevent pro-eryptotic and hemolytic effects and are promising candidate compounds for ameliorating eryptosis-associated diseases.