Quantitative determination of casein genetic variants in goat milk: Application in Girgentana dairy goat breed

Abstract

The study was conducted to develop a high-performance liquid chromatographic (HPLC) method to quantify casein genetic variants (s2-, β-, and κ-casein) in milk of homozygous individuals of Girgentana goat breed. For calibration experiments, pure genetic variants were extracted from individual milk samples of animals with known genotypes. The described HPLC approach was precise, accurate and highly suitable for quantification of goat casein genetic variants of homozygous individuals. The amount of each casein per allele was: s2-casein A=2.9 ± 0.8 g/L and F=1.8 ± 0.4 g/L; β-casein C=3.0 ± 0.8 g/L and C1=2.0 ± 0.7 g/L and κ-casein A=1.6 ± 0.3 g/L and B=1.1 ± 0.2 g/L. A good correlation was found between the quantities of s2-casein genetic variants A and F, and β-casein C and C1 with other previously described method. The main important result was obtained for κ-casein because, till now, no data were available on quantification of single genetic variants for this protein.