Combination of multiplex PCR and PCR-DGGE for monitoring common sourdough-associated Lactobacillus species
- Authors: SETTANNI, L; VALMORRI, S; VAN SINDEREN, D; SUZZI, G; PAPARELLA, A; CORSETTI, A
- Publication year: 2006
- Type: Articolo in rivista (Articolo in rivista)
- Key words: PCR multiplex, DGGE, impasti acidi
- OA Link: http://hdl.handle.net/10447/37449
Abstract
A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.