Premature termination codon 124 derivatives as a novel approach to improve the read-through of premature amber and ochre stop codons

Abstract

Nucleotide changes within an exon may alter the trinucleotide normally encoding a particular amino acid, such that a new stop signal is transcribed into the mRNA open reading frame. A recent approach to directly overcome the deleterious effects caused by nonsense mutations is represented by readthrough strategies which take advantage of the known properties of aminoglycosides that can suppress stop codons.