Novel protese from marine organisms with potential interest in restoration procedures

Abstract

In the last decades molecular biology allowed the development of innovative protocols in the field of conservation/restoration of cultural assets. In this work we presents novel hydrolyses isolated from marine invertebrate organisms, whose protease activity was previously tested in laboratory by zymography on polyacrilamide gel (in presence of 0.1 of gelatin) Very interesting these enzymes are activated/work in a range of temperatures between 4 ° C and 37 ° C. In this study two sets of proteases were applied, to bio-clean works of art surfaces, at the environments temperature (19° to 25.5° C). Before remove, the water-soluble components of the layers have been analyzed by high-pressure size molecular exclusion chromatography (SEC-HPLC, BioSuite 250 to 10 μm SEC 7.5 x 300 mm Waters), followed by electrophoresis on acrylamide gel (SDS-PAGE 15%) and silver staining of the bands. The results reveal the presence of protein molecules in concentrations between 24 and 110 μg/m, and molecular weight between 20-35 kDa and 60-90 kDa. The enzymatic cleaning showed skillful results, without providing the heating of the enzyme solution or of the surface on which they were applied. The particular feature makes these protease more appropriate then the other, which usually are activated at temperatures >37 ° C. Our hypothesis is that these enzymes will implement the efficiency of enzymatic cleaning protocols, according to the conservative restoration procedures.