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BRUNO GIUSEPPE PIGNATARO

Luminometric Sub-nanoliter Droplet-to-droplet Arrays (LUMDA-chip) for Drug Screening on Phase I Metabolism Enzymes

  • Authors: Arrabito, G; Galati, C; Castellano, S; Pignataro, B.
  • Publication year: 2013
  • Type: Articolo in rivista (Articolo in rivista)
  • OA Link: http://hdl.handle.net/10447/74599

Abstract

Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for amultiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. themost relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.