High-Resolution In Vivo Identification of miRNA Targets by Halo-Enhanced Ago2 Pull-Down
- Authors: Li, Xiaoyi; Pritykin, Yuri; Concepcion, Carla P; Lu, Yuheng; La Rocca, Gaspare; Zhang, Minsi; King, Bryan; Cook, Peter J; Au, Yu Wah; Popow, Olesja; Paulo, Joao A; Otis, Hannah G; Mastroleo, Chiara; Ogrodowski, Paul; Schreiner, Ryan; Haigis, Kevin M; Betel, Doron; Leslie, Christina S; Ventura, Andrea
- Publication year: 2020
- Type: Articolo in rivista
- OA Link: http://hdl.handle.net/10447/667859
Abstract
Li, Pritykin, Concepcion et al. report the development of Halo-enhanced Ago2 pull-down (HEAP), a method that streamlines the experimental identification of Ago2-miRNA-mRNA interaction sites in murine cells and tissues.