Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain
- Authors: Ciarlet, M.; Hoffmann, C.; Lorusso, E.; Baselga, R.; Cafiero, M.; Banyai, K.; Matthijnssens, J.; Parreno, V.; DE GRAZIA, S.; Buonavoglia, C.; Martella, V.
- Publication year: 2008
- Type: Articolo in rivista (Articolo in rivista)
- Key words: Rotavirus, gastroenteritis, genotype
- OA Link: http://hdl.handle.net/10447/36486
Abstract
An ovine rotavirus (OVR) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in Zaragoza, Spain, and the VP4, VP7, VP6, NSP4, and NSP5/NSP6 genes were subsequently characterized molecularly. Strain OVR762 was classified as a P[14] rotavirus, as the VP4 and VP8* trypsin-cleavage product of the VP4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the P11[14] human rotavirus (HRV) strain PA169, isolated in Italy. Analysis of the VP7 gene product revealed that OVR762 possessed G8 serotype specificity, a type common in ruminants, with the highest degree of aa identity(95–98%) shared with serotype G8 HRV, bovine rotavirus, and guanaco (Lama guanicoe) rotavirus strains. Moreover, strain OVR762 displayed a bovine-like NSP4 (genotype E2) and NSP5/NSP6 (genotype H3), and a VP6 genotype I2, as well as a long electropherotype pattern. This is the first report of a lamb rotavirus with P[14] and G8 specificities, providing additional evidence for the wide genetic and antigenic diversity of group A rotaviruses.