ALCOHOLIC LIVER DISEASE: A MOUSE MODEL REVEALS PROTECTION BY LACTOBACILLUS FERMENTUM

Abstract

Objectives: Alcoholism is one of the most devasting diseases with high incidence, but knowledge of its pathology and treatment is still plagued with gaps mostly because of the inherent limitations of research with patients. we developed an animal model for studying liver histopathology, Hsp /heat-shock protein)-chaperones involvemnent, and response to treatment. Methods: The system was standardized using mice to which ethanol was orally administered alone or in combination with lactobacillus fermentum following a precise schedule over time and applying, at predetermined intervals, a battery of techniques (histology, immunohistochemistry, western blotting, real-time PCR, immunoprecipitation, 3-nitrosyne labelling) to assess liver pathology (e.g., steatosius, fibrosis), and Hsp60 and iNOS (inducible form of nitric oxide synthase) gene expression and protein levels, and post-translational modifications. Results: Typical ethanol-induced liuver pathology occurred and the effect of the probiotic coulkd be reliably monitored. steatosis score, iNOS levels, and nitrate proteins (e.g. Hsp60) decreased after probiotic intake. Conclusions: We describe a mouse model useful for studying liver disease induced by chronic ethanol intake and for testing pertinent therapeutic agents, e.g., probiotics. we tested L. fermentum which reduced considerably ethanol-induced tissue damage and deleterious post-translational modifications of the chaperone Hsp60. The model is available to test other agents and probiotics with therapeutic potential in alcoholic liver disease.