Biochemical approaches to characterize targets responsible for acrylamide-induced inhibition of topoisomerase II

Abstract

Vinyl monomer acrylamide (AA), generally used in numerous industrial applications, has been classified by the International Agency for Research on Cancer (IARC) as “probably carcinogenic to humans” (group 2A), but the molecular mechanism underlying its genotoxicity has not fully known. Previously, we observed that Acrylamide (AA) was able to antagonize in vivo the citotoxicity of well know poison etoposide suggesting that topoisomerase II (Topo II) activity was affected by AA. In the current studies we investigated the inhibitory activity of acrylamide toward topoisomerase II by performing tests in vitro.