Studies on the antioxidant activity of the essential oil and extract of Tunisian Tetraclinis articulata (Vahl) Mast. (Cupressaceae)
- Authors: Ben Jemia, M; Chaabane, S; Senatore, F; Bruno, M; Kchouk, M E
- Publication year: 2013
- Type: Articolo in rivista (Articolo in rivista)
- OA Link: http://hdl.handle.net/10447/97281
Abstract
This study analyses the chemical composition and in vitro antioxidant activity of both the essential oil and the 80% aqueous acetone extract of Tetraclinis articulata leaves. The GC-MS analysis of the essential oil identified 66 components that comprise 93.5% of the oil. The major constituents of the oil are: bornyl acetate (31.4%), -pinene (24.5%) and camphor (20.3%). Antioxidant activities of the samples were determined using four different test systems, namely DPPH, -carotene/linoleic acid, reducing power and metal chelating activity assay. Test results from the DPPH system showed the strongest radical scavenging activity was exhibited by the 80% aqueous acetone extract (IC50 5.5 mgmL-1), which was two times higher than the positive control (BHT). The amount of the total phenolics, flavonoids and condensed tannins was very high in the 80% aqueous acetone extracts. The correlation between the antioxidant activity potential and total phenolic level of the extract was noted