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YANA ALEEVA

Ultrathin and nanostructured ZnO-based films for fluorescence biosensing applications

  • Authors: Satriano, C; Fragalà, M; Aleeva, Y
  • Publication year: 2012
  • Type: Articolo in rivista (Articolo in rivista)
  • Key words: ZnO thin film; MOCVD-colloidal lithography; Biosensing; Protein adsorption; Fluorescence recovery after photobleaching
  • OA Link: http://hdl.handle.net/10447/97152

Abstract

The fluorescence-based sensing capability of ultrathin ZnO-SiO(2) nanoplatforms, deposited by an integrated approach of colloidal lithography and metal organic chemical vapor deposition, has been investigated upon adsorption of fluorescein-labeled albumin, used as model analyte biomolecule. The protein immobilization process after spontaneous adsorption/desorption significantly enhances the green emission of the different ZnO-based films, as evidenced by scanning confocal microscopy, corresponding to a comparable protein coverage detected by X-ray photoelectron spectroscopy. Moreover, experiments of fluorescence recovery after photobleaching evidence that the protein lateral diffusion at the biointerface is affected by the chemical and/or topographical patterning of hybrid ZnO-SiO(2) surfaces. The used approach is very promising for biomolecular detection applications of these ZnO-SiO(2) nanoplatforms, by simple sizing of the 2D vs. 3D patterning design, which in turn is accomplished by the fine tuning of the integrated colloidal lithography-chemical vapor deposition processes. (C) 2011 Elsevier Inc. All rights reserved.